It appears that your browser may be outdated and performance may be limited. The My Study builder is best viewed with the latest internet browsers. However, if you’d prefer to use our standard form, you can access it below.

Go to Internet Explorer Quick Quote Form

Dismiss this message

Salivary Interleukin-10

Salivary Cytokines Quick Start Research Guide

Download the de facto guide for grant applications, summaries, and research studies.

Need Help?

Ask an expert
Biomarkers

1. How to collect Salivary Interleukin-10

APPROVED SALIVARY CYTOKINE COLLECTION METHODS

Passive Drool Saliva Collection Kit

SalivaBio Passive Drool Method

Use With: Adults, Children 6+

Salivary Cytokine Collection Protocol

Collection volume, general considerations, and basic guidelines to maximize salivary IL-10 sample integrity. Use this analyte-specific collection protocol to plan you collection methodology and sampling schemes.

Biomarkers

2. How to Assay for Salivary Interleukin-10

Easy and accurate results from the most trusted Salivary Bioscience Laboratory.

All Lab Services

Order Code5209

Biomarkers

3. Technical Summary

Analyte Summary
Analyte: Interleukin-10
Aliases: IL-10, human cytokine synthesis inhibitory factor (CSIF)
Serum-Saliva Correlation: NA
Optimum Collection Volume: 100 μL
Assay Summary
Methodology: ECL
Sensitivity: 0.04 pg/mL
Assay Range: 0.04 – 1420 pg/mL
Assay Type: Quantitative

Background

Human interleukin-10 (IL-10). IL-10 is mainly produced by monocytes and some lymphocytes as a 20.5 kDa, glycosylated homodimeric protein. It is an anti-inflammatory cytokine that inhibits the synthesis of numerous cytokines (including IFN-γ, IL-2, IL-3, TNF-α, TNF-β, and GM-CSF) that suppress Th1 proinflammatory responses involved in promoting phagocytosis. Secretion is at very low levels in unstimulated tissues and IL-10 regulates its own expression in a negative feedback loop. IL-10 also functions to enhance B lymphocyte survival, proliferation and antibody production. Like IL-6, IL-10 is linked to the myokines, as exercise provokes an increase in circulating levels of IL-1ra, IL-10, and sTNF-R, suggesting that physical exercise fosters an environment of anti-inflammatory cytokines. IL-10 levels are moderately elevated during acute stress (1) and oral cancer (2).

References & Salivary Interleukin-10 Research

  1. Szabo YZ, Newton TL, Miller JJ, Lyle KB, Fernandez-Botran R. Acute stress induces increases in salivary IL-10 levels. Stress. 2016;19(5):499-505.
  2. Aziz S, Ahmed SS, Ali A, Khan FA, Zulfiqar G, Iqbal J, et al. Salivary Immunosuppressive Cytokines IL-10 and IL-13 Are Significantly Elevated in Oral Squamous Cell Carcinoma Patients. Cancer Invest. 2015;33(7):318-28.
  3. Rajendran P, Chen YF, Chen YF, Chung LC, Tamilselvi S, Shen CY, et al. The multifaceted link between inflammation and human diseases. Journal of cellular physiology. 2018;233(9):6458-71.
  4. Val M, Sidoti Pinto GA, Manini L, Gandolfo S, Pentenero M. Variations of salivary concentration of cytokines and chemokines in presence of oral squamous cell carcinoma. A case-crossover longitudinal prospective study. Cytokine. 2019;120:62-5.
  5. Wang X, Kaczor-Urbanowicz KE, Wong DT. Salivary biomarkers in cancer detection. Med Oncol. 2017;34(1):7.
  6. Huck O, Buduneli N, Bravo D. Inflammatory Mediators in Periodontal Pathogenesis. Mediators Inflamm. 2019;2019:2610184.
  7. Silva N, Abusleme L, Bravo D, Dutzan N, Garcia-Sesnich J, Vernal R, et al. Host response mechanisms in periodontal diseases. J Appl Oral Sci. 2015;23(3):329-55.
  8. Gaba FI, Sheth CC, Veses V. Salivary biomarkers and their efficacies as diagnostic tools for Oral Squamous Cell Carcinoma: Systematic review and meta-analysis. J Oral Pathol Med. 2018.
  9. Slavish DC, Graham-Engeland JE, Smyth JM, Engeland CG. Salivary markers of inflammation in response to acute stress. Brain, behavior, and immunity. 2015;44:253-69.
  10. Sheth CC, Lopez-Pedrajas RM, Jovani-Sancho MDM, Gonzalez-Martinez R, Veses V. Modulation of salivary cytokines in response to alcohol, tobacco and caffeine consumption: a pilot study. Sci Rep. 2018;8(1):16687.

How Can we help?

WordPress Cookie Plugin by Real Cookie Banner
Contact: Salimetrics (USA)
View All International Distributors

Test Your DBS samples with Salimetrics!

Experts in Saliva & Dried Blood Spot Testing for Research

The best science for your study

Learn More

OR CALL 800.790.2258

X
Collaboratory